THE DETERMINATION OF INORGANIC PHOSPHATE AND CREATINE PHOSPHATE IN TISSUE EXTRACTS
نویسندگان
چکیده
منابع مشابه
The determination of inorganic phosphate in the presence of labile phosphate esters.
Labile phosphate esters such as phosphocreatine, acetyl phosphate, and ribose-l-phosphate (1) have assumed great biological importance. These esters are so unstable that they are split with great rapidity by the reagents commonly used for inorganic phosphate determination. Therefore, the usual inorganic phosphate measurements in tissue extracts, etc., actually represent the sum of the inorganic...
متن کاملThe Determination of Inorganic Phosphate in Urine by Alkalimetric Titration,
In an investigation of the excretion of phosphate and other substances in short periods (chiefly of 1 hour) now being conducted in this laboratory, it has been necessary to make many inorganic phosphate determinations with limited quantities of urine. In most instances the accuracy possible with colorimetric and nephelometric methods has been sufficient, and, after a considerable amount of time...
متن کاملThe Determination of Inorganic Phosphate in Urine by Alkalimetric Titration,
In an investigation of the excretion of phosphate and other substances in short periods (chiefly of 1 hour) now being conducted in this laboratory, it has been necessary to make many inorganic phosphate determinations with limited quantities of urine. In most instances the accuracy possible with colorimetric and nephelometric methods has been sufficient, and, after a considerable amount of time...
متن کاملA stability-Indicating HPLC Method for Simultaneous Determination of Creatine Phosphate Sodium and its Related Substances in Pharmaceutical Formulation
The objective of the study was to develop a simple, specific and stability-indicating HPLC method for the simultaneous determination of creatine phosphate sodium (CPS) and its related substances in pharmaceutical formulation. Separation of creatine phosphate sodium from its major process impurities and degradation products was achieved on a Hypersil BDS C18 column (250 × 4.6 mm, 5 μm) with an a...
متن کاملA stability-Indicating HPLC Method for Simultaneous Determination of Creatine Phosphate Sodium and its Related Substances in Pharmaceutical Formulation
The objective of the study was to develop a simple, specific and stability-indicating HPLC method for the simultaneous determination of creatine phosphate sodium (CPS) and its related substances in pharmaceutical formulation. Separation of creatine phosphate sodium from its major process impurities and degradation products was achieved on a Hypersil BDS C18 column (250 × 4.6 mm, 5 μm) with an a...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1956
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65147-6